complementary dna cdna templates (Thermo Fisher)

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Thermo Fisher complementary dna cdna templates
Complementary Dna Cdna Templates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/template+complementary+dna/DNA/pmc12552602-140-0-18
Average 99 stars, based on 1 article reviews

complementary dna cdna templates - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Identification of miR-106b-5p as a senolytic miRNA
Article Snippet: The concentration and purity of RNA were determined using a NanoDrop spectrophotometer (Thermo Fisher). .. Template complementary DNA was synthesised from total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) or TaqMan MicroRNA Reverse Transcription (Applied Biosystems) according to the manufacturer's protocols. .. Real-time PCR was performed using Powerup SYBR Green Master Kit (Applied Biosystems) for mRNA or TaqMan Fast Advanced master mix (Applied Biosystems) for miRNA. miRNA expression was normalised to that of U6 snRNA and mRNA expression was normalised to GAPDH and 18S ribosomal RNA.

Article Title: Neuronal Defects in a Human Cellular Model of 22q11.2 Deletion Syndrome
Article Snippet: .. Total RNA was isolated using the RNeasy mini kit and RNase–Free DNase set (Qiagen), and template complementary DNA was prepared by reverse transcription using the SuperScript III First–Strand Synthesis SuperMix for RT–qPCR (Life Technologies). qPCR was performed with SYBR Green (Roche) on a ViiA7 machine (Applied Biosystems, Life Technologies). .. Data were processed using the QuantStudio RT–qPCR software (v1.1, Applied Biosystems).

Article Title: Identification of miR-106b-5p as a senolytic miRNA.
Article Snippet: The concentration and purity of RNA were determined using a NanoDrop spectrophotometer (Thermo Fisher). .. Template complementary DNA was synthesised from total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) or TaqMan MicroRNA Reverse Transcription (Applied Biosystems) according to the manufacturer’s protocols. .. Real-time PCR was performed using Powerup SYBR Green Master Kit (Applied Biosystems) for mRNA or TaqMan Fast Advanced master mix (Applied Biosystems) for miRNA. miRNA expression was normalised to that of U6 snRNA and mRNA expression was normalised to GAPDH and 18S ribosomal RNA.

Isolation:

Real-time Polymerase Chain Reaction:

SYBR Green Assay:

Synthesized:

Article Title: Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model
Article Snippet: The concentration and purity of RNA were determined using nanodrop. .. Template complementary DNA was synthesized from total RNA using the SuperScript II First-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. Real-time qRT-PCR analysis was performed on QuantStudio 1 (Thermo Scientific, Pleasanton, CA, USA) using POWER SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the manufacturer’s instructions.

Article Title: Tumor necrosis factor-inducible gene 6 protein and its derived peptide ameliorate liver fibrosis by repressing CD44 activation in mice with alcohol-related liver disease
Article Snippet: The concentration and purity of RNA were determined using nanodrop. .. Template complementary DNA was synthesized from total RNA using the SuperScript First-strand Synthesis System (Invitrogen) according to manufacturers’ instructions. .. We performed the real-time qRT-PCR analysis by using Power SYBR Green Master Mix (Applied Biosystem) on the manufacturers’ specifications (QuantStudio 1; Thermo Scientific).

Article Title: Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model.
Article Snippet: The concentration and purity of RNA were determined using nanodrop. .. Template complementary DNA was synthesized from total RNA using the SuperScript II First-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocols. .. Real-time qRT-PCR analysis was performed on QuantStudio 1 (Thermo Scientific, Pleasanton, CA, USA) using POWER SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the manufacturer’s instructions.

Article Title: Formyl peptide receptor 2 determines sex-specific differences in the progression of nonalcoholic fatty liver disease and steatohepatitis
Article Snippet: The concentration and purity of RNA were determined using nanodrop. .. Template complementary DNA was synthesized from total RNA using the SuperScript First-strand Synthesis System (Invitrogen) according to manufacturers’ instructions. .. We performed the real-time qRT-PCR analysis by using Power SYBR Green Master Mix (Applied Biosystem) on the manufacturers’ specifications (QuantStudio 1; Thermo Scientific).

Article Title: Tumor necrosis factor-inducible gene 6 protein and its derived peptide ameliorate liver fibrosis by repressing CD44 activation in mice with alcohol-related liver disease.
Article Snippet: The concentration and purity of RNA were determined using nanodrop. .. Template complementary DNA was synthesized from total RNA using the SuperScript First-strand Synthesis System (Invitrogen) according to manufacturers’ instructions. .. We performed the real-time qRTPCR analysis by using Power SYBR Green Master Mix (Applied Biosystem) on the manufacturers’ specifications (QuantStudio 1; Thermo Scientific).

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